Effects of Angiotensin I - Converting Indonesian Dried - salted fish on Blood Enzyme Pressure Inhib ; tory Substances Deriyed from of Rats
نویسندگان
چکیده
Indonesian dried-salted fish (DSF) was produced from skipjack tuna by soaking the flesh in 15% NaCl (DSF D or 25% NaCl (DSF II). The DSFs were then hydrolyzed by trypsin, chymotrypsin, Pronase E, and pepsin. Angiotensin I-conyerting enzyme (ACE) inhibitory actiyity was measured. The pepsin digest showed the highest inhibitory activity (IC,,; O.63 mg proteinlml). DSF II hydrolysate had higher inhibitory actiyity than that in DSF I. A three-month storage period of DSF gave higher ACE-inhibitory activity than that of 6 months. An oral administration of pepsin hydrolysate significantly decreased the blood pressure of rats. From the purification steps, at least 4 inhibitor peptides were found. The amino acid sequences of the peptides were Yal-Ala-Trp-Lys-Leu, Trp-Ser-Lys-Val-Val-Leu, Ser-Lys-Yal-Pro-Pro, and Cys-TrpLeu-Pro-Val-Tyr, with an ICs, yalue of 31.97, 156.28, 74.22, and 22.20 "M, respectiyely.
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